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Source:Lvli Release Time:2011-12-26 00:46
Assay : UV 1 , reagents and apparatus for improved methanol -benzaldehyde HP8453 UV-visible spectrophotometer 1cm cuvette 2 , reference solution accurately weighed reference substance (CoCl2 6H2O) 0.6g in 25ml volumetric flask , add water
Assay: UV
1, reagents and apparatus for improved methanol-benzaldehyde HP8453 UV-visible spectrophotometer 1cm cuvette 2, reference solution accurately weighed reference substance (CoCl2 · 6H2O) 0.6g in 25ml volumetric flask, add water to dissolve, set the volume to the mark, shake, using 1cm cuvettes was measured at 515 ± 2nm absorbance of Acocl2 · 6H2O. 3, the test solution for test precision that take 200mg in 10ml volumetric flask, add methanol, ultrasonic 30min, after cooling to room temperature, dilute to the mark, filtered, the filtrate precision drawing 2ml in 10ml flask, add methanol, 3ml, improved on-benzaldehyde 5ml, Mesa, shake, water bath for 2 hours, cool to room temperature, with 1cm cuvette, was measured at 515 ± 2nm absorbance a sample, while subject to the blank. 4, calculated concentration = (A sample × Wcocl2 · 6H2O) / (Acocl2 · 6H2O × 2 × W sample × 100%)
1, reagents and apparatus for improved methanol-benzaldehyde HP8453 UV-visible spectrophotometer 1cm cuvette 2, reference solution accurately weighed reference substance (CoCl2 · 6H2O) 0.6g in 25ml volumetric flask, add water to dissolve, set the volume to the mark, shake, using 1cm cuvettes was measured at 515 ± 2nm absorbance of Acocl2 · 6H2O. 3, the test solution for test precision that take 200mg in 10ml volumetric flask, add methanol, ultrasonic 30min, after cooling to room temperature, dilute to the mark, filtered, the filtrate precision drawing 2ml in 10ml flask, add methanol, 3ml, improved on-benzaldehyde 5ml, Mesa, shake, water bath for 2 hours, cool to room temperature, with 1cm cuvette, was measured at 515 ± 2nm absorbance a sample, while subject to the blank. 4, calculated concentration = (A sample × Wcocl2 · 6H2O) / (Acocl2 · 6H2O × 2 × W sample × 100%)
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